치아 발생과정에서 Smad4의 역할
Smad4 Mediated TGF-β/BMP Signaling in Tooth Formation Using Smad4 Conditional Knockout Mouse
윤지영, 백진아, 조의식, 고승오,
소속 상세정보
윤지영 ( Yoon Chi-Young ) - 전북대학교 치의학전문대학원 구강악안면외과학교실
백진아 ( Baek Jin-A ) - 전북대학교 치의학전문대학원 구강안악면외과학교실
조의식 ( Cho Eui-Sic ) - 전북대학교 치의학전문대학원 구강악안면외과학교실
고승오 ( Ko Seung-O ) - 전북대학교 치의학전문대학원 구강안악면외과학교실
KMID : 1036320130350020073
Abstract
Purpose: Smad4 is a central mediator for transforming growth factor-${\beta}$ 수식 이미지/bone morphogenetic protein ($TGF-{\beta}/BMP$ 수식 이미지) signals, which are involved in regulating cranial neural crest cell formation, migration, proliferation, and fate determination. Accumulated evidences indicate that $TGF-{\beta}/BMP$ 수식 이미지 signaling plays key roles in the early tooth morphogenesis. However, their roles in the late tooth formation, such as cellular differentiation and matrix formation are not clearly understood. The objective of this study is to understand the roles of Smad4 in vivo during enamel and dentin formation through tissue-specific inactivation of Smad4.
Methods: We generated and analyzed mice with dental epithelium-specific inactivation of the Smad4 gene (K14-Cre:$Smad4^{fl/fl}$ 수식 이미지) and dental mesenchyme-specific inactivation of Smad4 gene (Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지).
Results: In the tooth germs of K14-Cre:$Smad4^{fl/fl}$ 수식 이미지, ameloblast differentiation was not detectable in inner enamel epithelial cells, however, dentin-like structure was formed in dental mesenchymal cells. In the tooth germs of Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지 mice, ameloblasts were normally differentiated from inner enamel epithelial cells. Interestingly, we found that bone-like structures, with cellular inclusion, were formed in the dentin region of Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지 mice.
Conclusion: Taken together, our study demonstrates that Smad4 plays a crucial role in regulating ameloblast and odontoblast differentiation, as well as in regulating epithelial-mesenchymal interactions during tooth development.
키워드
TGF-beta/BMP;SMAD4;Odontoblast;Ameloblast;Tooth development
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